FIG. 8.
K105D, EWK289A3, and RK344A2 complement the tsBN67 cell proliferation defect. (A) Hamster tsBN67 cells were stably transfected with 1 μg of pCGNHCF-1N902 wild type, P134S, K105D, EWK298A2, and RK344A2. Following transfection, cells were incubated at 39.5°C with G418 for 2 weeks, and proliferating (rescued) colonies were stained with 0.5% crystal violet. (B) Quantitation of the number of rescued colonies after 2 weeks of selection at the nonpermissive temperature. Numbers are expressed relative to the value for wild-type HCF-1 (pCGNHCF-1N902).