Figure 2.

Overall treatment viability of antral mucosal explants ± SEM collected from porcine stomachs (n = 8) and cultured for 72 h in sterile DMEM containing 100 U/L penicillin and 100 μl/L streptomycin, 1 ml/L amphotericin, and 5% fetal bovine serum that was refreshed daily. Viability was assessed by calcein (10 μM) and ethidium homodimer-1 (20 μM) staining at 72 h. CO (CO_stim and CO_non; n = 14) explants treated with sterile PBS. BL (BL_stim and BL_non; n = 14), explants treated with a blank gastric digest. DF (DF_stim and DF_non; n = 12), explants treated with a simulated gastric digest of G's Formula^™ composed of dried cabbage, carrot, hemp, and oat flour. There was no significant effect of treatment, stimulation, or treatment by stimulation interactions.