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. 2021 Oct 4;8:684203. doi: 10.3389/fvets.2021.684203

Table 1.

Porcine stomachs (n = 3) were collected from a local abattoir and 4-mm punch biopsies of antral mucosal explants were cultured over 120 h in sterile DMEM containing 100 U/L penicillin and 100 μl/L streptomycin, 1 ml/L amphotericin, and 5% fetal bovine serum that was refreshed daily.

Hour % Viability SEM
0 97.5a 0.16
6 97.4a 0.11
24 95.6b 0.23
48 94.4c 0.23
72 94.5c 0.22
96 93.4bcd 0.69
120 92.6d 0.38

% Viability was assessed by calcein (10 μM) and ethidium homodimer-1 (20 μM) staining at 0, 6, 24, 48, 72, 96, and 120 h.

Different superscripts represent time points that are significantly different (p ≤0.05).