Table 2.
Media gastrin (pg/ml/g tissue ww) as assessed by spectrophotometric assay of a commercial ELISA for porcine gastrin over time in porcine antral explants stimulated with either 10−5 M carbachol (_stim) or sterile PBS (_non).
| Treatment | Hour | Gastrin (pg/ml/g tissue ww) | SEM |
|---|---|---|---|
| CO_stim | 48 | 12,379a | 1,256.5 |
| 60 | 13,098a | 1,329.4 | |
| 72 | 16,426b | 1,492.8 | |
| CO_non | 48 | 13,171 | 1,397.3 |
| 60 | 11,557 | 1,226.1 | |
| 72 | 13,433 | 1,492.8 | |
| BL_stim | 48 | 13,718 | 1,333.3 |
| 60 | 11,626 | 1,130.0 | |
| 72 | 13,890 | 1,350.0 | |
| BL_non | 48 | 13,563 | 1,264.8 |
| 60 | 12,569 | 1,172.1 | |
| 72 | 13,335 | 1,243.6 | |
| DF_stim | 48 | 12,787a | 1,241.0 |
| 60 | 15,915b | 1,544.5 | |
| 72 | 16,303ab | 1,582.2 | |
| DF_non | 48 | 14,383 | 1,609.6 |
| 60 | 14,738 | 1,649.4 | |
| 72 | 14,248 | 1,594.5 |
Explants were taken with 4-mm punch biopsy and cultured in sterile DMEM containing 100 U/L penicillin and 100 μl/L streptomycin, 1 ml/L amphotericin, and 5% fetal bovine serum that was refreshed daily. CO_stim (n = 10)/CO_non (n = 10), explants treated with sterile PBS. BL_stim (n = 10)/BL_non (n = 13), explants treated with a blank gastric digest. DF_stim (n = 11)/DF_non (n = 9), explants treated with a simulated gastric digest of G's Formula™, composed of dried cabbage, carrot, hemp, and oat flour.
Different superscripts represent time points that are significantly different within treatments (p ≤0.05).