Table 3.
Overall media nitrite (μM/ml/g tissue ww) as assessed by spectrophotometric assay of the Griess reaction over time in porcine 4-mm punch biopsy antral explants cultured in sterile DMEM containing 100 U/L penicillin and 100 μl/L streptomycin, 1 ml/L amphotericin, and 5% fetal bovine serum and refreshed daily.
| Overall treatment | Hour | Media nitrite (μM/ml/g tissue ww) | SEM |
|---|---|---|---|
| CO | 48 | 481a | 8.6 |
| 60 | 452b | 8.2 | |
| 72 | 442b | 8.1 | |
| BL | 48 | 484 | 9.5 |
| 60 | 470 | 9.2 | |
| 72 | 471 | 9.2 | |
| DF | 48 | 488a | 10.6 |
| 60 | 472ab | 10 | |
| 72 | 459b | 9.8 |
CO (CO_stim and CO_non; n = 14), explants treated with sterile PBS. BL (BL_stim and BL_non; n = 14), explants treated with a blank gastric digest. DF (DF_stim and DF_non n = 12), explants treated with a simulated gastric digest of Gs Formula™, composed of dried cabbage, carrot, hemp, and oat flour.
Different superscripts represent time points that are significantly different within treatments (p ≤0.05).