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Experimental protocols. The procedure of hepatic RIPC was performed by a three-cycle of 5 min before establishing the I/R in the isolated perfused heart. All isolated hearts were subjected to 30-min ligation of the left anterior descending coronary artery, followed by 90 of reperfusion, except for the control group. In the control group, the Langendorff isolated heart perfusion was established in 150 min. I/R, ischemia/reperfusion; RIPC, remote ischemic preconditioning.
