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. 2021 Oct 4;9:750171. doi: 10.3389/fcell.2021.750171

FIGURE 5.

FIGURE 5

Visualization of fluorescently labeled engineered exosomes in tumor-bearing mice in vivo. BALB/c tumor bearing mice injected with each of the following: PBS (200 μL), unload exosomes (unload Exo; 30 μg), NC exosomes (NC Exo; 30 μg), and miR-317b-5b-loaded engineered exosomes (miR-317b-5b Exo; 30 μg). (A) In vivo miR-317b-5b-loaded engineered exosomes biodistribution in tumor-bearing mice by animal imaging. (B) miR-317b-5b-loaded engineered exosomes labeled with PKH26 were intravenously injected into mice bearing osteosarcoma tumors. Lung, liver, spleen, kidney, and tumor tissues were harvested after 3, 12, 24, and 48 h post injection for ex vivo imaging. The fluorescent signal of PKH26-labeled miR-317b-5b-loaded engineered exosomes detected using IVIS. ***P < 0.001 vs. NC Exo. Error bars represented SD. Data represented three independent experiments.