FIG. 3.

Bid induces Bax oligomerization. Isolated mitochondria from HeLa cells were incubated with 1 μM Bid for 15 min at 30°C, and the mitochondrial pellet was treated with two different cross-linkers as described in Materials and Methods. After cross-linking, the mixture was immunoprecipitated with anti-Bax monoclonal antibody and analyzed by Western blotting with an anti-Bax polyclonal antibody.