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Antibodies used for standard Western blotting.
| Concentration | Species | |
|---|---|---|
| Primary antibody | ||
| Anti-Actin (Abcam, polyclonal) | 1:1000 | Rabbit |
| Anti-HIF-1α (Cayman, polyclonal) | 1:1000 | Rabbit |
| Anti-hydroxy-HIF-1α (Pro564) (D43B5) XP® (Cell signaling, monoclonal) | 1:500 | Rabbit |
| Secondary antibody | ||
| Anti-rabbit HRP conjugate (Sigma) | 1:100 000 | Goat |
Concentrations implicate antibody dilution in 0.1% TBS-T.
