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Active immune protection against A. hydrophila and P. fluorescens in zebrafish. Zebrafish were separately immunized with DNA vaccines and challenged by A. hydrophila with 4 × 105 CFU/fish and by P. fluorescens with 1.6 × 106 CFU/fish. The formula of calculating the RPS index is as below: 1 - (% vaccinated mortality/%non-vaccinated mortality) × 100.
