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. 2021 Oct 4;8:715183. doi: 10.3389/fnut.2021.715183

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Copyright © 2021 Gabbia, Carpi, Sarcognato, Cannella, Colognesi, Scaffidi, Polini, Digiacomo, Esposito Salsano, Manera, Macchia, Nieri, Carrara, Russo, Guido and De Martin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of OC on α-SMA (A, B) and COL1A1 (C, D) mRNA and protein expression in LX2 cell line. *P < 0.05, ****P < 0.0001 vs. control, ^ϕP < 0.05, ^ϕϕP < 0.01, ^ϕϕϕP < 0.001 vs. TGF-β1 treated cells. Representative images of α-SMA and COL1A1-related red fluorescence are shown. Cell nuclei are stained blue with DAPI. At least 8 fields per coverslip have been analyzed to obtain the quantification of fluorescence. Results are the mean of 3 independent experiments and have been analyzed by one-way ANOVA followed by the Tukey's post hoc test.