Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 4;12:750200. doi: 10.3389/fmicb.2021.750200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Carranza, Menguiano, Valenzuela-Gómez, García-Cazorla, Cabezón and Arechaga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Visualization of transconjugant cells. MG1655 donor cells hosting R388:trwBmKate2 (A) or R388:trwCmKate2 (B) were mixed with UB1637 recipient cells expressing mCitrine. Conjugation was performed in enriched M9 minimal media plates and then, cells were transferred to the microscope for image-recording. Images were recorded at different emission wavelengths (540 and 630 nm, for mCitrine and mKate2, respectively) at the same location, in order to discriminate donor, recipient and transconjugant cells. Merged images show the transconjugant cells in orange (right panel). These transconjugants are UB1637 cells expressing mCitrine that, after receiving the R388 plasmid, have started to express TrwBmKate2 (A) or TrwCmKate2 (B), encoded by the plasmid.