Table 1.
Assay performance of the t0 and per-well method
| Assay performance characteristics | t0 Method | Per-Well Method | Relative Reduction by Per-Well Method |
|---|---|---|---|
| Sample rejection rate (n rejected/n total) | 13.44% (68/506) | 4.74% (24/506) | 65% |
| Intra-assay CVa | 5.28% | 4.48% | 15% |
| Uncorrected interassay CV for PC1 | 16.74% | 11.88% | 29% |
| Uncorrected interassay CV for PC2 | 16.34% | 11.55% | 29% |
| Corrected interassay CV for PC1b | 13.58% | 7.85% | 42% |
| Corrected interassay CV for PC2b | 15.29% | 9.86% | 36% |
| Relative reduction interassay CV for PC1 by correctionb | 19% | 34% | — |
| Relative reduction interassay CV for PC2 by correctionb | 6% | 15% | — |
PC, positive controls consist of two samples (PC1 and PC2).
a
Excluding rejected samples.
b
Corrected for interassay differences using four additional controls as described in the Materials and Methods section.