Table 3.
Routine immunological evaluation at baseline.
| Pt1 | Pt2 | Pt3 | Pt4 | Pt5 | Pt6 | Pt7 | Pt8 | Pt9 | Pt10 | Pt11 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Hb (mg/dl) | 15.1 | 16.9 | 15.5 | 14.3 | 15.2 | 12.9 | 14.1 | 15.4 | 15.2 | 12.2 | 14.4 |
| PLT (10x3/ml) | 123 | 174 | 214 | 245 | 129 | 228 | 259 | 165 | 218 | 253 | 192 |
| WBC (10x3/ml) | 9.07 | 6.22 | 7.6 | 5.39 | 4.06 | 9.01 | 4.76 | 4.42 | 5.79 | 3.73 | 4.52 |
| Eosinophils (% and 10x3/ml) | 1.3/120 | 2.9/180 | 2.5/190 | 0.4/20 | 4.4/180 | 7.3/660 | 1.3/60 | 2.3/100 | 5.7/330 | 1.1/40 | 3.5/220 |
| Total lymphocytes (10x3/ml) | 1.05 | 2.76 | 2.6 | 2.7 | 1.6 | 4.8 | 2.2 | 1.2 | 2.3 | 1.2 | 1.1 |
| CD3+ (% and cell/µl) | 87/913 | 81/2235 | 83/2158 | 64.7/1746 | 77/1232 | 86/4128 | 58/1276 | 70/840 | 70/1610 | 73/876 | 82.7/909 |
| CD4+ (% and cell/ µl) | 46/483 | 65/1794 | 36/936 | 37/999 | 27/432 | 38/1824 | 36.7/807 | 48/576 | 37/851 | 40.6/487 | 39.7/436 |
| CD8+ (% and cell/ µl) | 41/430 | 15/414 | 44/1144 | 24/648 | 45/720 | 31.7/1521 | 17/374 | 20/240 | 25/575 | 17/204 | 42.1/463 |
| CD19+ (% and cell/µl) | 5.5/52 | 9/248 | 6.7/174 | 17/459 | 12/192 | 6.3/302 | 26/572 | 18/216 | 17/391 | 19.8/237 | 8/88 |
| CD16+56+ (% and cell/µl) | 6.5/68 | 8.8/243 | 9.9/257 | 15.6/421 | 7/112 | 7.8/374 | 8.7/191 | 11/132 | 9/207 | 12.2/146 | 6.1/66 |
| CD3+CD4+CD27+CD45RO- Naïve T cell (%) | 26 | 68.6 | 52.9 | 64.2 | 41.9 | 79 | 70.5 | 66.5 | 70 | 27.2 | 48 |
| CD3+CD4+CD27+CD45RO+ Tcm (%) | 49.9 | 25.4 | 33.2 | 25.9 | 49.1 | 12.2 | 22.2 | 26.8 | 21.7 | 68.6 | 41.1 |
| CD3+CD4+CD27-CD45RO+ Tem (%) | 4.52 | 1.43 | 1.14 | 1.98 | 2.54 | 3.79 | 1.13 | 1.94 | 2.73 | 0.31 | 1.88 |
| CD3+CD4+CD27-CD45RO- Temra (%) | 17.2 | 2.37 | 8.74 | 6.08 | 5.5 | 3.93 | 3.11 | 2.99 | 3.54 | 2.73 | 6.59 |
| CD3+CD4+CD27+CD45RO+CXCR5+ pTfh (%) | 28.9 | 24.9 | 18.7 | 24.3 | 45.5 | 8.22 | 23.3 | 12.8 | 17 | 53.9 | 30.3 |
| CD27-IgD- Double negative B cells (%) | 16.5 | 0.86 | 2.11 | 6.61 | 1.13 | 3.77 | 2.58 | 8.29 | 8.62 | 7.48 | 1.56 |
| CD27+IgD+ Unswitched memory B cells (%) | 3.46 | 0.39 | 0.56 | 11.9 | 3.46 | 4.99 | 0.85 | 9.73 | 11.8 | 14 | 2.23 |
| CD27+IgD- Switched memory B cells (%) | 13.8 | 0.054 | 0.25 | 8.11 | 0.13 | 5.5 | 1.26 | 5.19 | 5.49 | 4.16 | 0.83 |
| CD27-CD21+ Naïve B cells (%) | 50.4 | 96.7 | 85.1 | 66.4 | 83.6 | 34.6 | 91.5 | 63 | 62.6 | 37.6 | 90.5 |
| CD27-CD21- TLM B cells (%) | 27.1 | 1.75 | 13.5 | 5.59 | 10.2 | 49 | 5.22 | 13 | 10.8 | 33.9 | 4.27 |
| CD21+ (RM) Switched memory B cells (%) | 76.2 | 67.9 | 70.3 | 84.6 | 72.5 | 94.2 | 84.7 | 88.1 | 90.1 | 62.9 | 70.3 |
| CD21- (AM) Switched memory B cells (%) | 19.5 | 32.1 | 26.6 | 10.4 | 26.4 | 4.79 | 13.2 | 8.62 | 7.86 | 33 | 27.7 |
| IgG (mg/dl) | 1090 | 993 | 723 | 1420 | 1132 | 1132 | 1165 | 1159 | 698 | 1180 | 708 |
| IgA (mg/dl) | 33 | 50 | 22 | 5 | 5 | 356 | 113 | 45 | 91 | 290 | 45 |
| IgM (mg/dl) | 10 | 7 | 55 | 131 | 5 | 284 | 45 | 23 | 24 | 25 | 23 |
| IgE (kU/L) | 1 | 1.67 | 9.94 | 142 | 1 | 2.5 | 2 | 3 | 207 | 2.47 | 1 |
| IgG anti-tet | NA | NA | NA | R | NR | R | R | TR | R | NA | TR |
| IgG anti-pneumo | NA | NA | NA | R | NR | R | R | TR | R | NA | TR |
| TCR α/β | NA | 96.5% | 98.3% | 96.5% | 89.6% | 78.2% | 91.6% | 96.4% | 80.8% | 89.6% | 96% |
| TCR γ/δ | NA | 3.3% | 1.5% | 3% | 8.6% | 21.5% | 6.4% | 1.2% | 17.7% | 10.2% | 3.6% |
| continued | Pt12 | Pt13 | Pt14 | Pt15 | Pt16 | Pt17 | Pt18 | Pt19 | Pt20 | Pt21 | |
| Hb (mg/dl) | 13.4 | 13.3 | 13.8 | 12.1 | 15.6 | 13.8 | 14.2 | 16.3 | 13 | 12.9 | |
| PLT (10x3/ml) | 241 | 169 | 339 | 218 | 232 | 109 | 129 | 173 | 84 | 128 | |
| WBC (10x3/ml) | 3.81 | 4.82 | 7.95 | 7.6 | 5.15 | 7.00 | 5.73 | 4.99 | 2 | 4.61 | |
| Eosinophils (% and 10x3/ml) | 0.5/20 | 1.7/80 | 1.5/120 | 2.2/170 | 2/100 | 1.2/80 | 1.9/108 | 2/100 | 0.7/14 | 4/0.18 | |
| Total lymphocytes (10x3/ml) | 0.8 | 1.6 | 2.1 | 2.0 | 1.8 | 2290 | 1.19 | 1.65 | 0.51 | 0.83 | |
| CD3+ (% and cell/µl) | 75/600 | 61.3/981 | 90.6/1902 | 88.5/1770 | 85/1530 | 90.6/2074 | 87/1035 | 83.7/1981 | 96.6/493 | 70.9/586 | |
| CD4+ (% and cell/ µl) | 51/408 | 35.2/563 | 53.7/1127 | 57/1140 | 30/540 | 54/1236 | 50.3/599 | 41.8/690 | 56.2/287 | 43.5/361 | |
| CD8+ (% and cell/ µl) | 17/136 | 20/320 | 29.7/623 | 25.5/510 | 43/774 | 32.5/744 | 32/381 | 24.4/403 | 35.2/179 | 25/207 | |
| CD19+ (% and cell/µl) | 10.5/84 | 11.3/176 | 3.9/82 | 9.8/196 | 6.3/113 | 1.4/32 | 3.1/178 | 9.4/155 | 0.2/1 | 13.4/111 | |
| CD16+56+ (% and cell/µl) | 11.6/93 | 26.2/419 | 5.1/107 | 3.7/74 | 7.5%/135 | 7.4/169 | 9.8/561 | 6.2/102 | 2.7/14 | 14.4/119 | |
| CD3+CD4+CD27+CD45RO- Naïve T cell (%) | 55.9 | 46.2 | 24.5 | 36.9 | 51.1 | 68.8 | 26.5 | 81 | 49.4 | 32.2 | |
| CD3+CD4+CD27+CD45RO+ Tcm (%) | 38.6 | 47.5 | 46 | 44.3 | 23.8 | 24.4 | 56.7 | 16.2 | 37.6 | 57.3 | |
| CD3+CD4+CD27-CD45RO+ Tem (%) | 0.088 | 0.12 | 0.5 | 2.64 | 0.61 | 0.47 | 0.97 | 1.46 | 1.21 | 0.37 | |
| CD3+CD4+CD27-CD45RO- Temra (%) | 3.22 | 2.72 | 26.6 | 12.9 | 23.4 | 4.74 | 13.4 | 0.55 | 10.2 | 8.46 | |
| CD3+CD4+CD27+CD45RO+CXCR5+ pTfh (%) | 30.2 | 38.1 | 19.2 | 23.4 | 18.5 | 15 | 30.2 | 15.1 | 14.7 | 28 | |
| CD27-IgD- Double negative B cells (%) | 5.42 | 5.79 | 9.72 | 15.5 | 3.87 | 3.85 | 1.2 | 3.27 | 33.3 | 4.77 | |
| CD27+IgD+ Unswitched memory B cells (%) | 4.18 | 4.9 | 6.63 | 3.31 | 3.57 | 0.41 | 3.16 | 2 | 0 | 6.57 | |
| CD27+IgD- Switched memory B cells (%) | 2.26 | 1.52 | 12.7 | 10 | 0.84 | 0.36 | 0.094 | 0.8 | 0 | 0.72 | |
| CD27-CD21+ Naïve B cells (%) | 82.1 | 74.7 | 57.2 | 62.9 | 85.2 | 91.6 | 69.4 | 87.9 | 33.3 | 31.9 | |
| CD27-CD21- TLM B cells (%) | 6.32 | 13.4 | 15.9 | 14.4 | 7.39 | 5.22 | 23.1 | 5.89 | 33.3 | 53.7 | |
| CD21+ (RM) Switched memory B cells (%) | 72 | 77.9 | 88.8 | 74 | 81.3 | 73.3 | 50 | 66.9 | 0 | 79.1 | |
| CD21- (AM) Switched memory B cells (%) | 21 | 16.3 | 8.71 | 19.4 | 15.7 | 13.3 | 50 | 21 | 0 | 19.4 | |
| IgG (mg/dl) | 1021 | 1197 | 655 | 618 | 1052 | 876 | 325 | 726 | 746 | 289 | |
| IgA (mg/dl) | 5 | 16 | 550 | 96 | 5 | <4 | <4 | 91 | <4 | 17 | |
| IgM (mg/dl) | 8 | 34 | 87 | 189 | 25 | <5 | 10 | 87 | <5 | 37 | |
| IgE (kU/L) | 1 | 1 | 3.78 | 211 | 92 | NA | NA | NA | 2 | NA | |
| IgG anti-tet | R | R | NA | NA | NR | NA | NA | R | NA | TR | |
| IgG anti-pneumo | NR | NR | NA | R | NR | NA | NA | R | NA | R | |
| TCR α/β | 97.5% | 93.4% | 92.5% | 97% | 87.6% | NA | 96.9% | 83.6% | 94.6% | 92.3% | |
| TCR γ/δ | 3.3% | 5.3% | 5.9% | 2.8% | 12.1% | NA | 2.3% | 9.8% | 3.4% | 2.3% |
WBC, White blood cells; PLT, Platelets; HB, Hemoglobin; Tcm, central memory T cells; Tem, effector memory T cells; Temra, terminally differentiated effector memory T cells; pTfh, peripheral follicular helper memory T cells; TLM, tissue like memory B-cells; RM, resting memory B-cells; AM, activated memory B-cells.
NA, Not available; NR, Not Responder; R, Responder; TR, Transient Responder.
Serum immunoglobulin concentrations from Whelan MA et al., J. Clin Immunol 2006 (24); T-cell subsets from Schatorie E.J.H. et al., Clin Immunol 2011 (25); B-cell subsets from Piatosa B. et al., Cytometry part B, Clinical Cytometry 2010 and Duchamp M et al., Immunity, Inflammation and Disease 2014 (26, 27); Regulatory T-cell subsets from van Gent R. et al., Clinical Immunology 2009 (28).