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. 2021 Sep 15;40(20):e107237. doi: 10.15252/embj.2020107237

Figure EV5. BAK α1‐2 loop cleavage does not inhibit cytochrome c release.

Figure EV5

  1. FLAG‐BAK or FLAG‐BAKthrombin were stably expressed in Bax −/− Bak −/− MEFs and assessed by immunoblotting.
  2. Cells from A were treated with combined BH3 mimetic drugs for 24 h and cell death was analysed by PI uptake. Data are mean ± SD of three independent experiments.
  3. Mitochondria‐enriched fractions isolated from cells in A were incubated with cBID (10 nM) in the presence or absence of thrombin for the indicated times and membrane pellet (P) and supernatant (S) were separated and immunoblotted for cytochrome c or BAK (4B5). Data from two independent experiments are shown, third experiment is shown in Fig 5E.

Source data are available online for this figure.