Fig. 1.

Illustration of the workflow to obtain histological images in traditional histopathology and Deep-PAM. Biological tissue (e.g., a mouse brain) is harvested and cut by hand. The top pathway shows the two traditional methods to obtain H&E-stained histological images of thin sections from FFPE and frozen section, respectively. The bottom pathway illustrates our proposed method using UV-PAM imaging and deep-learning-based virtual staining that can directly and rapidly get virtual H&E-stained histological images (i.e., Deep-PAM) of fresh thick tissue.