Fig. 5.

UV-PAM and Deep-PAM validation using a 7-µm thick frozen section of a mouse brain. a–c, UV-PAM, Deep-PAM, and traditional H&E-stained image of a frozen sectioned slice. d–f, Zoomed-in UV-PAM, Deep-PAM, and BF-H&E images of the green regions in a–c, respectively, showing the ability of Deep-PAM to reveal individual cell nuclei. g–i, Zoomed-in UV-PAM, Deep-PAM, and BF-H&E images of the red regions in a–c, respectively, showing the ice crystal pitfalls. j–l, Zoomed-in UV-PAM, Deep-PAM, and BF-H&E images of the orange regions in a–c, respectively, showing the over-staining problem of hematoxylin in frozen section. m–o, Zoomed-in UV-PAM, Deep-PAM, and BF-H&E images of the blue regions in a–c, respectively, showing the under-staining problem of hematoxylin in frozen section. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)