UV-PAM and Deep-PAM validation by capturing the surface of a hand-cut 2-mm thick and fresh mouse brain. a and b, UV-PAM and Deep-PAM images of the thick and fresh mouse brain’s surface. c, BF-H&E image of the layer next to the fresh mouse brain’s surface for ground truth comparison. d and e, UV-PAM and Deep-PAM images of the cortex region (green regions in a and b) close to the corpus callosum in the fresh brain. f, BF-H&E image of the cortex region (green region in c) next to corpus callosum in the adjacent layer. g and h, UV-PAM and Deep-PAM images of the cortex region (red regions in a,b) in the fresh brain. i, BF-H&E image of the cortex region (red region in c) in the adjacent layer. j,k, UV-PAM and Deep-PAM images of the canduputamen region (blue regions in a and b) in the fresh brain. l, BF-H&E image of the canduputamen region (blue region in c) in the adjacent layer. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)