Reagent or Resource	Source	Identifier
Antibodies
Anti‐Prok2	Abcam	Cat# ab76747; RRID: AB_1524238
Anti‐ProkR2	Alomone Labs	Cat #APR‐042
Alexa Fluor 488 Goat Anti‐Rabbit antibody	Thermo Fisher Scientific	A‐11008; RRID: AB_143165
Alexa Fluor 568 Goat Anti‐Rabbit antibody	Thermo Fisher Scientific	A‐11011; RRID: AB 143157
Vectashield HardSet Mounting Medium with DAPI	Vector Laboratories	Cat# H‐1500; RRID:AB_2336788
Deposited Data
AAV1.pProk2. Cre.T2A.mCherry	VectorBuilder	#169013
AAV1.pProkr2. Cre.T2A. Venus	VectorBuilder	#169014
Mouse suprachiasmatic nucleus scRNASeq data	This paper	GSE167927
Chemicals, Peptides, and Recombinant Proteins
Triton x‐100	Sigma Aldrich	Cat #X100
Eagle’s basal medium	Sigma Aldrich	Cat# B1522
Dulbecoo Modified Eagle Medium	Sigma Aldrich	Cat# D5796
B27	GIBCO	Cat# 17504‐044
HEPES	Sigma Aldrich	Cat# H0887
FCS	Sigma Aldrich	Cat# 59665
EBSS	GIBCO	Cat# 24010043
D‐glucose	Fisher	Cat# 10373242
Glutamax	GIBCO	Cat# 35050061
MK801	Sigma Aldrich	Cat# M107
APV	Sigma Aldrich	Cat# A5282
D‐MEM	Sigma Aldrich	Cat# D5030
NaHCO3	Fisher	Cat# 10553325
Heat inactivated horse serum	GIBCO	Cat# 16050130
Recombinant Prokineticin 2	Sigma Aldrich	Cat# SRP3146
Recombinant PKRA7	Tocris, Biotechne	Cat# 6238
Critical Commercial Assays
10× Genomics Chromium Single Cell Library Kit v2	10× Genomics	Cat# 120234
RNAscope Fluorescent Multiplex	Advanced Cell Diagnostics	Cat# 320850
RNAscope Probe‐ Mm‐ Prok2	Advanced Cell Diagnostics	Cat# 447941
RNAscope Probe‐ Mm‐ Avp	Advanced Cell Diagnostics	Cat# 401391
RNAscope Probe‐ Mm‐ Avp‐C2	Advanced Cell Diagnostics	Cat# 401391‐C2
RNAscope Probe‐ Mm‐Prokr2‐C3	Advanced Cell Diagnostics	Cat# 498431‐C3
RNAscope Probe‐ Mm‐Vipr2‐C2	Advanced Cell Diagnostics	Cat# 465391‐C2
RNAscope Probe‐ Mm‐Avpr1a‐C2	Advanced Cell Diagnostics	Cat# 418061‐C2
Worthington Papain dissociation kit	Worthington/ Lorne Labs	Cat# LK003150
Experimental Models: Organisms/Strains
PER2::LUC (B6.129S6‐Per2tm1Jt/J)	Jax Laboratories	RRID:IMSR_JAX:006852
Cry1−/−, Cry2−/−	Van der Horst et al, 1999	N.A.
Recombinant DNA
pAAV. Syn.NES.jRCaMP1a.WPRE.SV40	Addgene	RRID : A ddgene_100848
pAAV. Syn.GCaMP6f.WPRE.SV40	Addgene	RRID : A ddgene_100837
pAAV‐EF1a‐DIO‐Chr2(H113R)‐EYFP‐HGHpA	Addgene	RRID : A ddgene_20298
Software and Algorithms
Cell Ranger analysis pipeline v2.0	10× Genomics	https://support.10xgenomics.com/single‐cell‐gene‐expression/software/pipelines/latest/what‐is‐cell‐ranger; RRID:SCR_017344
Loupe Browser	10× Genomics	https://support.10xgenomics.com/single‐cell‐gene‐expression/software/visualization/latest/what‐is‐loupe‐cell‐browser; RRID:SCR_018555
STAR aligner v2.5.2b	Dobin et al, 2013	STAR; RRID:SCR_015899
BioDare	Moore et al, 2014	https://www.biodare.ed.ac.uk
GraphPad Prism 8.0	GraphPad Software	http://www.graphpad.com/; RRID:SCR_002798
R version 3.6.1	R Foundation for Statistical Computing, Vienna, Austria	http://www.R‐project.org; RRID:SCR_001905
R package “circular” version 0.4‐93	Comprehensive R Archive Network	https://r‐forge.r‐project.org/projects/circular
R package “ggplot2” version 3.1.2 and “scales” version 1.0.0	Comprehensive R Archive Network	https://cran.r‐project.org/web/packages/ggplot2/index.html; RRID:SCR_014601
R package “RColorBrewer” version 1.1‐2	Comprehensive R Archive Network	http://colorbrewer2.org/
RStudio version 1.2.1335	RStudio Team, Boston, MA	http://www.rstudio.com; RRID:SCR_000432
Matlab R2020b	MathWorks Inc, USA	http://www.mathworks.com/products/matlab/; RRID:SCR_001662
Matlab Toolbox “CircularGraph” version 2.0.0.0	P. Kassebaum (GitHub)	Retrieved February 3, 2021; https://github.com/paul‐kassebaum‐mathworks/circularGraph
FIJI/ImageJ	Schindelin et al, 2012	https://imagej.net/Fiji; RRID:SCR_002285
ZEN Blue	Zeiss	RRID :S CR_013672
Adobe Illustrator CC	Adobe	RRID :S CR_010279