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Validation of the interaction between nanobodies (including site‐directed mutants) and mCherry by isothermal titration calorimetry (ITC) at 25°C. (a) LaM2 series nanobodies (wild type, S44A, W67A, S68A, and W119A/Y120A) and (b) LaM4 series nanobodies (wild type, N103K, N103D, N108K, and N108D)
