FIGURE 4.

Isothermal titration calorimetry (ITC) and fluorescence‐based size exclusion chromatography (F‐SEC) assays confirmed that LaM2 and LaM4 can bind simultaneously to mCherry. (a) The ITC result of LaM2 binding to the LaM4‐mCherry preformed complex. (b) The ITC result of LaM4 binding to the LaM2‐mCherry preformed complex. (c) The F‐SEC results of mCherry (red), LaM2‐mCherry (light green), LaM4‐mCherry (dark green), and the premixed LaM2‐mCherry complex mixed with LaM4 (blue). The right panel shows an enlargement of 14–17 ml of F‐SEC to clearly show the change in protein complex size