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. 2021 Sep 17;40(20):e107680. doi: 10.15252/embj.2021107680

Figure EV5. RASSF1C promotes invasion via EV transfer in vivo .

Figure EV5

  1. Quantification of the size of the T47DDsRed cells within MDA‐MB‐231CFP;Cre;Control or MDA‐MB‐231CFP;Cre;HA‐RASSF1C tumors indicating equal contribution to tumor volume.
  2. Representative 3 h migration paths of T47DeGFP reporter cells within a single intravital imaging field of tumors co‐injected with MDA‐MB‐231CFP;Cre;Control (Control, left) or MDA‐MB‐231CFP;Cre;HA‐RASSF1C (RASSF1C, right).
  3. Representative confocal images of lungs of mice with mammary gland tumors initiated by either the co‐injection of T47DDsRed reporter cells with MDA‐MB‐231CFP;Cre;Control or MDA‐MB‐231CFP;Cre;HA‐RASSF1C. Scale bars represent 200 μm.
  4. Quantification of the number of T47DDsRed metastatic events found in the lungs of mice with mammary gland tumors initiated by MDA‐MB‐231CFP;Cre;Control (Control) or MDA‐MB‐231CFP;Cre;HA‐RASSF1C (RASSF1C) cells. Whole lung tile images were used for quantification, 6 of 10 μm sections each, 100 μm apart.

Data information: Data are analyzed by Student’s t‐test and represented as mean ± SEM.