Table 1.

PNA-peptide conjugates used and MIC values

PNA	Sequence incl. BPP (5’-3’)	Target in mRNA sequence	MIC (μM)
PNA5561 (NrdA-PNA)	H-(KFF)3K-eg1-ATG TAT GTC G-NH2	CAGGUACGACAUACAUGAAUCAG	4
PNA5645 (2-base mismatch-PNA)	H-(KFF)3K-eg1-ATT TAT GGC G-NH2	NA	8
PNA5866 (4-base mismatch-PNA)	H-(KFF)3K-eg1-ATTGAT TGC G -NH2	NA	16
PNA 5562	H-(KFF)3K-eg1-ATG TCG TAC C-NH2	CAGGUACGACAUACAUGAAUCAG	8
PNA5563	H-(KFF)3K-eg1-TGT ATG TCG T-NH2	CAGGUACGACAUACAUGAAUCAG	8

NA, not applicable.

Sequences of NrdA-targeting PNA-peptides, their binding positions on mRNA target and their corresponding MIC values. The nrdA start codon is highlighted in bold, the PNA binding position is double underlined and mismatch bases are underlined. PNA-peptides were synthesized as described previously.⁴⁸