TABLE 3.
Filamentation of RIM101 pathway mutantsa
| Strain | Genotype | Plasmidc | pH | % Filamentation |
|---|---|---|---|---|
| BWP17b | RIM101/RIM101 | 4 | <0.25 | |
| BWP17 | RIM101/RIM101 | 8 | 65 | |
| DAY2 | RIM101/rim101− | 4 | <0.5 | |
| DAY2 | RIM101/rim101− | 8 | 80 | |
| DAY5 | rim101−/rim101− | 4 | <0.25 | |
| DAY5 | rim101−/rim101− | 8 | <0.25 | |
| DAY44 | rim101−/rim101− | pRIM101 | 4 | 1 |
| DAY44 | rim101−/rim101− | pRIM101 | 8 | 90 |
| DAY96 | rim101−/rim101− | pRIM101-405 | 4 | <0.5 |
| DAY96 | rim101−/rim101− | pRIM101-405 | 8 | 70 |
| DAY90 | RIM101/RIM101 | pRIM101-405 | 4 | <0.5 |
| DAY90 | RIM101/RIM101 | pRIM101-405 | 8 | 70 |
| DAY23 | rim20−/rim20− | 4 | <0.25 | |
| DAY23 | rim20−/rim20− | 8 | <0.25 | |
| DAY93 | rim20−/rim20− | pRIM101-405 | 4 | 5 |
| DAY93 | rim20−/rim20− | pRIM101-405 | 8 | 70 |
| DAY111 | rim20−/rim20− | pRIM101 | 4 | <0.5 |
| DAY111 | rim20−/rim20− | pRIM101 | 8 | <0.5 |
| DAY61 | rim8−/rim8− | 4 | <0.25 | |
| DAY61 | rim8−/rim8− | 8 | <0.25 | |
| DAY62 | rim8−/rim8− | pRIM101-405 | 4 | 5 |
| DAY62 | rim8−/rim8− | pRIM101-405 | 8 | 70 |
| DAY114 | rim8−/rim8− | pRIM101 | 4 | <0.5 |
| DAY114 | rim8−/rim8− | pRIM101 | 8 | <0.5 |
| BWP17 | RIM101/RIM101 | (Serum) | 90 | |
| DAY5 | rim101−/rim101− | (Serum) | 90 | |
| DAY23 | rim20−/rim20− | (Serum) | 90 | |
| DAY61 | rim8−/rim8− | (Serum) | 80 |
a
Filamentation of strains in TC199 buffered at pH 4 or pH 8 for 4 h or incubated in serum for 2 h. Percent filamentation is rounded to the nearest 5%. Standard deviations range from 15% for samples that filament >65% to <5% for samples that filament ≤5%.
b
This strain is wild type for RIM20 and RIM8 as well.
c
Refers to the relevant genotype of the plasmid integrated into the Candida genome. pRIM101 is pDDB52, and pRIM101-405 is pDDB71.