TABLE 4.
Growth rates
| Strain | Genotype | pH | Doubling timea (h) |
|---|---|---|---|
| BWP17 | Wild type | 4 | 1.95 |
| BWP17 | Wild type | 8 | 3.1 |
| DAY5 | rim101−/rim101− | 4 | 1.9 |
| DAY5 | rim101−/rim101− | 8 | 2.3 |
| DAY23 | rim20−/rim20− | 4 | 2.05 |
| DAY23 | rim20−/rim20− | 8 | 2.45 |
| DAY61 | rim8−/rim8− | 4 | 2.05 |
| DAY61 | rim8−/rim8− | 8 | 2.65 |
a
Average doubling times of strains grown in TC199 medium buffered at pH 4 or pH 8 from two experiments were determined by measurements of the opitcal density at 600 nm (OD600) (values vary by ≤25%). OD600 was determined following shift to fresh TC199 medium and monitored continually over 4 h. These results include the samples used for the Northern blot analyses in Fig. 4 and 5.