Figure 3. MEXs^LPS was the main inducer of inflammatory polarization in macrophages in the cell culture supernatant.

(A) Uptake of exosomes by THP-1-derived macrophages. Exosomes were labeled with Exo-PHK26 (red), macrophages were labeled with 488-Phalloidin (green) and nuclei of macrophages with DAPI (blue), bar = 50 μm. (B) Transcription levels of TNF-α, IL-1β and IL-6 in THP-1-derived macrophages educated by conditioned media (Control), LPS-tMSCs CM, exosome supernatant or conditioned media without exosomes from LPS-tMSCs CM for 24 h. Data were represented as the mean ± SD of three independent experiments, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.