Figure 6. TSP-1 promotes differentiation of Th1 and Th17 cells and inhibits Tregs differentiation.

(A) Activated CD4⁺T cells were co-cultured with MEXs^LPS and MEXs^LPS-TSP1sh for 12hr, the percentage of Th1 (IFNγ⁺ CD4⁺T) cells was analyzed by flow cytometry. A representative image (left), statistical analysis of the data (right). (B) The percentage of Th17 (IL17⁺CD4⁺T) cells as analyzed by flow cytometry. (C) The percentage of Th2 (IL4⁺CD4⁺T) cells as analyzed by flow cytometry. (D) The percentage of Tregs (Foxp3⁺CD25⁺T) as analyzed by flow cytometry. Data are expressed as the mean ± SD from three experiments, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.