Skip to main content
. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Cancer Gene Ther. 2021 Apr 16;29(5):558–572. doi: 10.1038/s41417-021-00334-4

Figure 2. PIM3 knockout decreased growth over time in hepatoblastoma cells and inhibited their progression through the cell cycle.

Figure 2.

(A) PIM3 knockout (KO) cells exhibited a slower growth rate compared to HuH6 wild-type (WT) cells over the course of 96 hours. (B) Doubling time was significantly longer for PIM3 KO cells compared to HuH6 WT cells. Data represent at least three biologic replicates and are reported as mean ± standard error of the mean. (C) HuH6 WT or PIM3 KO cells were stained with propidium iodide and were analyzed using flow cytometry to evaluate progression through the cell cycle. FlowJo software was used for analysis. Quantification of average percent cells in each phase of the cell cycle across all replicates revealed a significant increase in G1 phase and a decrease in S phase in PIM3 KO cells compared to HuH6 WT cells. (D) Representative histograms showing percentages of one experiment. (E) Tabular results (mean percent cells in phase ± SEM) of the cell cycle analysis from three biologic replicates are provided.