Skip to main content
. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Cancer Gene Ther. 2021 Apr 16;29(5):558–572. doi: 10.1038/s41417-021-00334-4

Figure 4. PIM3 re-introduction rescued the anti-proliferative and anti-migratory phenotype observed with PIM3 knockout.

Figure 4.

(A) PIM3 knockout (KO) cells were transfected with an empty vector (EV) control or PIM3 cDNA expressing plasmid. Immunoblotting confirmed both absence of PIM3 protein expression in PIM3 KO cells and rescue of expression in PIM3 KO-PIM3 cDNA (PIM3 rescue). (B) Introduction of PIM3 cDNA in PIM3 KO cells returned proliferation to almost baseline levels. (C) Migration was also evaluated. Introduction of PIM3 cDNA in PIM3 KO cells returned migration to baseline levels. Representative images of migration inserts are presented to the bottom of the graph. Scale bars represent 100 μm. EV transfected controls did not affect (B) proliferation or (C) migration of PIM3 KO cells. Data represent at least three biologic replicates and are reported as mean ± standard error of the mean. NS: non-significant.