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. 2021 Oct 15;11:20513. doi: 10.1038/s41598-021-99979-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of GFP after intrathecal injection. AAV9/GFP was intrathecally injected via suboccipital puncture into 1-year-old and 6-week-old MLD mice. Representative images of immunostaining for GFP in sagittal sections of brain (a), spinal cord and dorsal root ganglia (DRG) (c). Boxes indicate the approximate regions from which the higher magnification images shown in (b) were acquired. (b) 1and 7, hypothalamus (HT); 2 and 8, subiculum (S); 3 and 9, inferior colliculus (IC); 4 and 10, brain stem ventral (BSV); 5 and 11, cerebellum (Cb); 6 and 12, spinal trigeminal tract (SpV). Sections were taken 3 or 13.5 months after injection of AAV9/GFP. Bar: 200 μM.