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. 2021 Oct 15;11:20513. doi: 10.1038/s41598-021-99979-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of transduced cells in the brain and spinal cord of treated MLD mice at 1 year of age. Immunostaining for GFP and the neuronal marker NeuN (a), the astrocyte marker GFAP (b), the Purkinje marker calbindin (c), the basket marker parvalbumin in the cerebellum (d), the microglia marker Iba1 (g) in the brain, and ChAT-positive motor neurons (e) and NeuN-positive neurons (f) in the cervical spinal cord, especially the ventral horn. Bar: 200 μM. Panels on the right highlight the observed co-localization.