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. 2021 Oct 15;11:20513. doi: 10.1038/s41598-021-99979-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Therapeutic effects of intrathecal injection. Motor function was assessed using a balance beam test with 15-month-old MLD mice left untreated (closed bars, n = 6), treated with AAV9/ARSA at 1 year (gray bars, n = 5), 6 weeks of age (open bars, n = 4) or wild type (WT) (diagonally hatched bars, n = 8). (a) Latency: the time required to traverse each beam. (b) Slips: the number of times the hind feet of mice slipped off a beam. All animals were given three trials on the beams. *p < 0.03.