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. 2021 Oct 15;11:16866. doi: 10.1038/s41598-021-96351-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Toxicity studies in BBM cells. (A) The effect of lapatinib-loaded hiPSC-platelets against BBM cells at 24, 48, and 72 h, with various concentrations of lapatinib loaded into the hiPSC-platelets. The inset table (bottom) shows the IC₅₀ (µM) of lapatinib-loaded hiPSC-platelets against BBM cells at 24, 48, and 72 h (mean ± SD, n = 3). (B) Cytotoxic effects of non-loaded hiPSC- and donor-derived platelets, free lapatinib, and lapatinib-loaded hiPSC- and donor-derived platelets on BBM1 and BBM2 cells at 24, 48, and 72 h. ** indicates p < 0.001. (C) Representative flow cytometry plots demonstrating the surface expression of Annexin-V on CD326 + BBM cells exposed to non-loaded and lapatinib-loaded hiPSC- and donor-derived platelets. (D) Surface Annexin-V quantification of BBM cells exposed to non-loaded and lapatinib-loaded hiPSC- and donor-derived platelets (mean ± SD, n = 3). ** indicates p < 0.001. (E) mRNA expression of apoptosis-associated genes in BBM cells under various treatment conditions, evaluated by TaqMan RT-qPCR. * indicates p < 0.001. (F) Representative flow cytometry plot showing BBM cells exposed to non-loaded, C6-loaded, and 1:1 lapatinib + C6-loaded hiPSC- and donor-derived platelets for 48 h. (G) CCK8 viability assay results showing the percentage of viable BBM cells after exposure to non-loaded, C6-loaded, and 1:1 lapatinib + C6-loaded hiPSC- and donor-derived platelets for 48 h (mean ± SD, n = 3). ** indicates p < 0.001.