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. 2021 Oct 15;11:20549. doi: 10.1038/s41598-021-99911-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of measurable proteins in normal DBS and serum samples in a quantitative multi-plex immunoassay. (A) Summary of the number of 1000 proteins that are below the LOD, within the detectable range, above the LOD, or that didn’t fall completely in either of the aforementioned categories in at least 2/3 of all samples assessed in DBS and serum, respectively. (B) Distribution of the concentration of measurable proteins in DBS and serum samples by violin plot. (C) Scatterplot of the distribution of the concentration of measurable proteins in DBS and serum samples. (D) Correlation of the 18 DBS and 28 Serum measurable FDA-approved protein biomarkers in at least 2/3 of the 72 DBS and 200 serum samples assessed with each other and reported serum literature values.