Figure 1.

Three CYPs are consistently and specifically upregulated by geranyl acetate exposure. (a) Schematic of RNASeq workflow. Flies were exposed for 5 h to either DMSO, 5% geranyl acetate (GA), or 5% ethyl lactate (EL). Three biological replicates of ~ 200 antenna were collected and used for RNASeq analysis. (b) A heat map showing the average expression in log₂(RPM) of each of the 42 antennal-expressed CYP genes in each odorant exposure condition (n = 3). GA-induced CYPs are in orange font. (c) The antennal expression of Cyp6d5 and Cyp4p1 detected with qRT-PCR following exposure to 5% geranyl acetate for varying lengths of time. Expression is normalized to expression at 0 h of exposure for each gene (n = 4). (d) The antennal expression of Cyp6d5 and Cyp4p1 detected with qRT-PCR following 2 h of exposure to varying concentrations of geranyl acetate in DMSO. Expression is normalized to DMSO exposure alone (n = 5–6). (e) The expression of six CYPs following 2-h exposure to 5% geranyl acetate and measured with qRT-PCR (n = 5). Data were analyzed by one-way ANOVAs followed by Tukey post-hoc tests. Statistical significance is presented as **p < 0.01, ***p < 0.001, and ****p < 0.0001. Other comparisons were not significant (p > 0.05). For (c-e), data are shown as the mean ± SEM.