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. 2021 Oct 15;11:20530. doi: 10.1038/s41598-021-99910-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pre-exposure to geranyl acetate reduces spiking responses to geranyl acetate. (a) SSR traces show responses of an ab5 sensillum to a 500 ms pulse of 0.01% and 0.1% geranyl acetate in control flies not exposed to geranyl acetate. (b) SSR traces show responses of an ab5 sensillum to geranyl acetate in control flies following two hours of exposure to 5% geranyl acetate. (c,d) Similar to (b), but for (c) Cyp4p1¹ and (d) Cyp6a8¹ mutants. (e) Quantification of spiking responses to geranyl acetate. Bar graphs depict the mean ± SEM overlaid with the individual data points. Data were analyzed with a two-way ANOVA followed by Tukey’s post-hoc test. Statistical significance is presented as *p < 0.05 and ****p < 0.0001. Other comparisons were not significant (p > 0.05).