Table 1.

Summary of genotypes of candidate mutations from WGS analysis and diagnostic tests

Breed*	Candidate mutations and their nucleotide positions (bp) on chromosome 1 based on GalGal6						Ml genotype†
	rs316201461	InDel1	rs312865584	InDel2	rs317670985	rs794497277
	93,851,717	93,852,277	93,853,513	93,857,288	93,865,772	93,866,099
White Crested Black Polish (n = 1)	Het	Mutant	Mutant	Mutant	Mutant	Mutant	Ml/−
All other Melanotic chickens (n = 58)	Mutant	Mutant	Mutant	Mutant	Mutant	Mutant	Ml/−
Red junglefowl (n = 1)	Het	WT	WT	WT	WT	Mutant	ml+/ml+
Red junglefowl (n = 2)	Het	WT	WT	WT	WT	WT	ml+/ml+
Red junglefowl (n = 1)	WT	WT	Het	WT	WT	Het	ml+/ml+
Partridge Cochin (n = 1)	Het	WT	WT	Het	Het	Het	ml+/ml+
Partridge Cochin (n = 1)	WT	WT	WT	Het	WT	WT	ml+/ml+
Nonmelanotic chickens (n = 6)	Het	WT	WT	WT	WT	WT	ml+/ml+
Nonmelanotic chickens (n = 11)	WT	WT	WT	WT	WT	Het	ml+/ml+
All other nonmelanotic chickens (n = 113)	WT	WT	WT	WT	WT	WT	ml+/ml+

^*Breeds that do not show periodic feather patterns like Buff, Columbian, and Black were not included in this comparison because their Ml genotype is unknown.

^† Inferred from plumage phenotype.