T cells in the islets of organ donors with and without T1D. (A) Numbers of CD4 (blue triangles) and CD8 (magenta inverse triangles) T cells detected per islet in each donor are shown. (B and C) Frequencies of TCR sequences detected from single and multiple cells are shown in green and white bars, respectively. White rectangles represent each unique TCR clonotype. Panels (B and C) show TCR sequencing results from CD4 and CD8 T cells, respectively. (D) Individual symbols (blue: CD4, magenta: CD8, filled triangles: T1D donors, and open triangles: nondiabetic donors) indicate Shannon diversity indexes of TCR repertoires, in which 1 represents each T cell expresses a different TCR clonotype from all other T cells in a sample, and 0 represents all T cells in a sample express an identical TCR clonotype. Statistical significance for numbers of CD4 and CD8 T cells per islet and Shannon Diversity Index of TCR repertoires were assessed by the Wilcoxon rank sum test.