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. 2021 Oct 5;118(41):e2107208118. doi: 10.1073/pnas.2107208118

Fig. 2.

Fig. 2.

TCR clonotypes with positive responses in the screening with preproinsulin and insulin DRiP truncated peptide pools. TCR transductant cell lines with an NFAT reporter were cultured with 103 preproinsulin (A and C) and 36 insulin DRiP (B) truncated peptide pools or an anti-CD3 antibody in the presence of autologous EBV-transformed B cells. TCR transductants express a ZsGreen-1 fluorescent protein when activated. Percentages of TCR transductant cells expressing ZsGreen-1 in response to each peptide pool or anti-CD3 antibody are plotted. Individual panels show screening results of each TCR transductant responding to one or more peptide pools derived from T1D organ donors (A and B) or nondiabetic control donors (C).