Table 2.
Preproinsulin (PPI) and insulin DRiP–reactive T cell receptors
| Donor ID | TCR ID | Freq | HLA | Epitope | EC50 (nM) | TRAV | TRAJ | CDR3a | TRBV | TRBJ | CDR3b |
| T1D-1 | 1.C8 | 5.3% | A0201 | PPI: 1-11/2-12/2-10 | 68 | TRAV24 | TRAJ58 | CAFKRETSGSRLTF | TRBV13 | TRBJ2-1 | CASSTRLAGDEQFF |
| T1D-1 | 1.F3 | 15.8% | A0201 | PPI: 2-12 | 848 | TRAV39 | TRAJ39 | CAVENAGNMLTF | TRBV10-2 | TRBJ2-1 | CASWTVSYNEQFF |
| T1D-1 | 1.B10-1 | 7.9% | A0201 | PPI: 15-23 | 332 | TRAV8-3 | TRAJ33 | CAVVADSNYQLIW | TRBV4-2/4-3 | TRBJ2-2 | CASSQTKGTGELFF |
| T1D-1 | 1.F1 | 5.3% | A0201 | PPI: 15-24 | 14,418 | TRAV39 | TRAJ41 | CAVSNSGYALNF | TRBV29-1 | TRBJ2-5 | CSVFHRGETQYF |
| T1D-1 | 1.E9-1 | 10.5% | B5001 | PPI: 92-99 | 18,470 | TRAV12-2 | TRAJ34 | CAVNIRYNTDKLIF | TRBV6-2/6-3 | TRBJ1-5 | CASSSIQGSGSGQPQHF |
| T1D-1 | 1.C1 | 10.5% | A0201 | DRiP: 1-9 | 3 | TRAV12-1 | TRAJ39 | CGENNAGNMLTF | TRBV27 | TRBJ2-5 | CASSLQPPGTSTETQYF |
| T1D-2 | 10.C6-1 | 2.9% | C1203 | PPI: 23-32 | 784 | TRAV19 | TRAJ39 | CALSGALNNAGNMLTF | TRBV27 | TRBJ2-5 | CASSLFGYRQETQYF |
| T1D-3 | 23.F9 | 3.9% | C0304 | PPI: 1-11 | 73 | TRAV12-3 | TRAJ48 | CAMSALGNFGNEKLTF | TRBV19 | TRBJ2-1 | CASSIAGGNEQFF |
| T1D-3 | 19.A1 | 2.6% | C0304 | PPI: 1-11 | 64 | TRAV8-4 | TRAJ11 | CAVSDQGSGYSTLTF | TRBV28 | TRBJ1-5 | CASSWTANQPQHF |
| T1D-3 | 20.E5 | 3.9% | C0304 | PPI: 1-11 | 66 | TRAV14/DV4 | TRAJ52 | CAMSNAGGTSYGKLTF | TRBV28 | TRBJ1-4 | CASSLARYNEKLFF |
| T1D-3 | 20.F1 | 3.9% | C0304 | PPI: 1-11 | 44 | TRAV14/DV4 | TRAJ43 | CAMRLHNNNDMRF | TRBV28 | TRBJ1-5 | CASIASRYNQPQHF |
| T1D-3 | 22.A10 | 1.3% | C0304 | PPI: 1-11 | 73 | TRAV8-1 | TRAJ13 | CAVNAAGGYQKVTF | TRBV28 | TRBJ2-1 | CASIPDRYNEQFF |
| T1D-3 | 23.H5 | 5.2% | A0201 | PPI: 3-13 | 3,992 | TRAV38-2/DV8 | TRAJ22 | CAYRSPARQLTF | TRBV6-1 | TRBJ2-7 | CASSEGWGVPSYEQYF |
| T1D-3 | 23.C12 | 2.6% | A0201 | PPI: 15-24/15-25 | 35,347 | TRAV41 | TRAJ42 | CAVSGGSQGNLIF | TRBV28 | TRBJ1-2 | CASSPPTGWGGYTF |
| T1D-3 | 20.G1 | 5.2% | C0304 | PPI: 69-77/69-79 | 525 | TRAV1-2 | TRAJ8 | CAVRMNTGFQKLVF | TRBV9 | TRBJ2-1 | CASSVGMDPGLGYNEQFF |
| T1D-4 | 28.D3 | 15.4% | A0201 | PPI: 31-41/34-41 | 753,461 | TRAV26-2 | TRAJ26 | CILTDNYGQNFVF | TRBV27 | TRBJ1-1 | CASSLIGLNTEAFF |
| T1D-4 | 28.E6 | 2.6% | A2902 | PPI: 46-54/47-54 | 85,759 | TRAV19 | TRAJ28 | CALSEAGAGSYQLTF | TRBV2 | TRBJ2-5 | CASSPSGTSSQETQYF |
| T1D-4 | 28.E11 | 5.1% | B1801 | PPI: 91-100 | 1,278 | TRAV12-2 | TRAJ49 | CAVSMNTGNQFYF | TRBV29-1 | TRBJ2-1 | CSVQVYNEQFF |
| T1D-5 | 54.F1 | 2.6% | A0101 | PPI: 96-103 | 10,519 | TRAV3 | TRAJ26 | CAVPDNYGQNFVF | TRBV7-2 | TRBJ2-2 | CASSLVVELFF |
| T1D-6 | 96.A9 | 3.4% | B0801 | DRiP: 1-9 | 83 | TRAV12-2 | TRAJ39 | CAVNVYNAGNMLTF | TRBV30 | TRBJ1-1 | CAWSVRGGSYMNTEAFF |
| T1D-6 | 96.F5 | 1.7% | A0201 | PPI: 3-11 | 108,241 | TRAV8-6 | TRAJ48 | CAVSDISNFGNEKLTF | TRBV9 | TRBJ2-3 | CASSVVGLGTDTQYF |
| T1D-6 | 93.D1 | 2.5% | A0201 | PPI: 15-25 | 4,487 | TRAV5 | TRAJ8 | CAVTKDTGFQKLVF | TRBV20-1 | TRBJ2-1 | CSARDHFGGSGYEQFF |
| T1D-6 | 96.B4 | 3.4% | C0501 | PPI: 91-99 | 168 | TRAV12-2 | TRAJ31 | CAVNNARLMF | TRBV6-5 | TRBJ2-1 | CASRPTSGGYNEQFF |
| T1D-7 | 86.C1 | 2.3% | B4102 | PPI: 91-100/92-100/92-102 | 13 | TRAV19 | TRAJ16 | CALSEAGFSDGQKLLF | TRBV19 | TRBJ2-1 | CASSIQFSYNEQFF |
| T1D-7 | 84.D9 | 1.6% | B4102 | PPI: 91-100/92-100/92-102 | 2 | TRAV29/DV5 | TRAJ43 | CAASNSNDMRF | TRBV7-9 | TRBJ2-1 | CASSLAQREQFF |
| T1D-7 | 86.G3-2 | 1.6% | B3501 | PPI: 94-102 | 84 | TRAV8-6 | TRAJ33 | CAVSDGYQLIW | TRBV6-1 | TRBJ2-7 | CASSGREAPYEQYF |
| ND-2 | 131.D5 | 2.2% | A2902 | PPI: 45-53 | 1,185,142 | TRAV12-2 | TRAJ35 | CAVNIGFGNVLHC | TRBV7-9 | TRBJ2-2 | CASSGGNTGELFF |