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. 2021 Oct 4;118(41):e2108276118. doi: 10.1073/pnas.2108276118

Fig. 5.

Fig. 5.

lnc-Cxcl2 inhibits Cxcl2 expression through ribonucleoprotein La. (A) Immunoblot analysis of proteins purified by lnc-Cxcl2 probes in MLE-12 cells infected with VSV (multiplicity of infection [MOI] = 1) for 12 h. (B) qPCR analysis of lnc-Cxcl2 level purified by La antibody in MLE-12 cells infected with VSV (MOI = 1) for 12 h. (C) Schematic illustration of La truncations (Top), and the immunoblot analysis of lnc-Cxcl2 and La truncations interaction in HEK293T cells transfected with Flag-tagged La truncations (Bottom). FL, full length. (D) qPCR analysis of Cxcl2, Ccl2, Ifnb1, and Tnf mRNA expressions in Ssb+/+ and Ssb−/− MLE-12 cells infected with IAV (MOI = 1) for 12 h. (E) qPCR analysis of the DNase I sensitivity of Cxcl2 and Ccl2 promoters in Ssb+/+ and Ssb−/− MLE-12 cells infected with VSV (MOI = 1) for indicated hours. Data are representative of three independent experiments (A and C) or three independent experiments with n = 3 biological replicates (B, D, and E; shown as mean + SEM), two-tailed unpaired Student’s t test. Ctrl, control; IB, immunoblot.