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Cell seeding density used for 3D cell cultures.
| Type of culture | PDAC cells | Cell density (cells/ml) | Stromal cells | Cell density (cells/ml) |
|---|---|---|---|---|
| Mono-culture | MIA PaCa-2 | 2.5 × 105 | CAFs and THP-1 | 1.0 × 105 (each) |
| PANC-1 | 2.5 × 105 | |||
| Triple-culture | MIA PaCa-2 | 0.5 × 105 | CAFs and THP-1 | 1.0 × 105 (each) |
| PANC-1 | 0.5 × 105 |
PDAC, pancreatic ductal adenocarcinoma; CAFs, cancer-associated fibroblasts.
