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Composition of medium used in this study
| Component | MM | BMM | BM | BMMY | LB |
|---|---|---|---|---|---|
| Methanol | 0.5% | 0.5% | 1% | 0.5% | – |
| YNB (Yeast Nitrogen Base) | 1.34% | 1.34% | – | 1.34% | – |
| Biotin | 4 × 10−5% | 4 × 10−5% | – | 4 × 10−5% | – |
| Potassium phosphate buffer, pH 6.0 | – | 100 mM | – | 100 mM | – |
| Yeast extract | – | – | 0.5% | 1% | 0.5% |
| Peptone | – | – | – | 2% | – |
| Tryptone | – | – | – | – | 1% |
| NaCl | – | – | – | – | 1% |
| 5 N NaOH | – | – | – | – | pH 7.0 |
MM: minimal methanol; BMM: buffered minimal methanol; BMMY: buffered methanol-complex medium
