Table 1.
Primer sequences used in this study
| Gene | Function | Forward primer (5′-3′) | Reverse primer (5′-3′) | Reference |
|---|---|---|---|---|
| DcBCH1 | Full lengths clone | ATGGCGGCCGGAATTTCGGCG | CTATGATCGGCTAGCTCTGGA | – |
| DcBCH1 | Overexpression vector conduction |
TTTACAATTACCATGGGATCCATGGCGGC CGGAATTTCGGCG |
ACCGATGATACGAACGAGCTCCTATGATCGGC TAGCTCTGGA |
– |
| AtGGPS | RT-qPCR | TGCTTGTGAACTCGTCGGAGGT | CACGGCGGAGATCGTCGTTATC | – |
| AtPSY | RT-qPCR | TGCTGCTCTCGCTGATACAGTTG | CCTCTTCTCGCATCTTCGCCTAC | Yin et al. 2020 [60] |
| AtPDS | RT-qPCR | GCTCAATGACGATGGCACGGTTA | CGGCATACACGCTCAGAAGGTTAC | Yin et al. 2020 [60] |
| AtZDS | RT-qPCR | GACATTCGCAACCTTGACAGCATAA | CCTTGAGCATACGCAACAGAGAAG | Yin et al. 2020 [60] |
| AtZISO | RT-qPCR | CTAACCTCACCTTAATCCGCCGTAT | AAGAGAACAACTCCAAGGACAACAC | Yin et al. 2020 [60] |
| AtCRTISO | RT-qPCR | TCCTCAGCATTCCAACCATTCTTGA | GCGAGTGTCCTTAGCCAACCAA | Yin et al. 2020 [60] |
| AtLCYE | RT-qPCR | GTAGTGTCAGAGCTAGCGGC | AAGGCTAAACCAGCAGGACC | – |
| AtLCYB | RT-qPCR | AGATGGAATGTGCCTTGTTGTTGGA | ACTCGGAGATGTTGATTGCGGTTC | Yin et al. 2020 [60] |
| AtLut1 | RT-qPCR | GCGTCTCTATCCTCATCCTCCT | GTATGGTTGCTCCTGTGGTCAT | – |
| AtCCD1 | RT-qPCR | TGGCAGCAGCATCATCTCAGTC | ATCACGGATGGGAGCGAAGTTG | – |
| AtCCD4 | RT-qPCR | CGGCACTCTTCCACTGTCACTT | TTCGGTTAATCGGACGGCGTAG | – |
| AtZEP | RT-qPCR | TCTTCGTTGACATTGCTATGCCATC | CGCCGCCTTCTTATCTGAACCA | Yin et al. 2020 [60] |
| AtNCED3 | RT-qPCR | GGAGATGGCTTGGTGGCAATCA | GCTTCTCGTGGCTGACAAGGAA | – |