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. 2021 Oct 4;118(41):e2113174118. doi: 10.1073/pnas.2113174118

Fig. 5.

Fig. 5.

Tubulation promotes phagocytosis and phagosome–lysosome fusion in macrophages. (A) Average number of phagosomes versus time in RAW 264.7 cells treated with dsDNA, LPS, or Tudor. Arrow shows zymosan addition (N = 30 cells). (B) Percentage TLs contacting phagosomes (% TL-P contact) (N = 50 cells,). (C) Phagosome–lysosome fusion (P-L fusion) given by mean G/R value in RAW 264.7 cells (n = 60 cells). (D) Schematic of fusion assay in Tudor-treated cells where lysosomes and phagosomes are labeled with Alexa 488 dextran (G) and pHrodo Red zymosan (R), respectively. (E) Number of phagosomes in Tudor-treated RAW 264.7 cells depleted of Arl8b (N = 100 cells). (F) Average number of phagosomes versus time in Pmac (M0) treated with dsDNA, Tudor, or LPS. Arrow shows zymosan addition (N = ∼30 cells). (G) Percentage TLs contacting phagosomes in M0 Pmacs (n = 30 cells). (H) Extent of phagosome lysosome fusion (P-L fusion) (N = 30 cells). (I) Lysosomes (G) and phagocytosed zymosan particles (R) with or without dsDNA or Tudor treatment in RAW 264.7 cells (J) Inset of TLs-P contacts indicated by #. ***P < 0.0005; *P < 0.05 (one-way ANOVA with Tukey post hoc test). n.s: nonsignificant; n = 100 phagosomes wherever mentioned.