(A) Aβ animals fed HB101 (gray) and HT115 (red) exhibited delayed paralysis compared to those on OP50 (black) (n ≥ 89).
(B) Aβ animals fed DA837 (blue) and OP50 (black) became paralyzed at the same time (n ≥ 183).
(C) Western analysis showed no effect of diet on Aβ accumulation. The Aβ monomer and dimer are indicated by asterisks (* and **, respectively); bracket shows Aβ oligomers. Additional replicates are in Figure S1F.
(D) ATP levels in Aβ C. elegans fed OP50 were reduced compared to wild type (WT); the HB101 diet increased ATP in Aβ animals (n ≥ 10).
(E) Representative images of mitochondria, visualized with TOMM-20∷RFP, in WT and Aβ animals. Scale bars, 10 μm.
(F) Average mitochondrial length was affected by Aβ accumulation and diet; individual measurements (gray symbols, n ≥ 30) indicated.
(G) Superoxide (O2−), measured with MitoSox, was higher in Aβ animals fed OP50 versus HB101 (n ≥ 11).
(H) Hydrogen peroxide (H2O2) level, quantified with Amplex red, was greater in both WT and Aβ nematodes given OP50 (n ≥ 7).
(I) H2O2, measured with H2DCFDA, was increased in WT and Aβ animals fed OP50 (n ≥ 8).
Error bars show SEM; *p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA with Dunnett’s post-test. See also Figures S1 and S2.