Antibodies |
anti-β-Amyloid 6E10 |
BioLegend |
Cat#803001; RRID: AB_2564653 |
anti-α-tubulin A11 |
Millipore Sigma |
Cat#T9026; RRID: AB_477593 |
Anti-NDUFS3 |
Abcam |
Cat#ab14711; RRID: AB_301429 |
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP |
ThermoFisher Scientific |
Cat#31430; RRID: AB_228307 |
Bacterial and virus strains |
Escherichia coli OP50 |
Caenorhabditis Genetics Center (CGC) |
N/A |
Escherichia coli HB101 |
CGC |
N/A |
Escherichia coli HT115 |
CGC |
N/A |
Escherichia coli DA837 |
CGC |
N/A |
Chemicals, peptides, and recombinant proteins |
D(+)-glucose |
Fisher Scientific |
Cat#: AC410955000 CAS: 50-99-7 |
Sodium Homogamma Linolenate |
Nu-Chek Prep |
Cat#: S-1143, CAS: 65881-87-0 |
Oleic Acid |
ThermoFisher Scientific |
Cat#:31997, CAS: 112-80-1 |
Methylcobalamin |
Millipore Sigma |
Cat#: M9756, CAS: 13422-55-4 |
L-methionine |
Fisher Scientific |
Cat#: AC166160025 CAS: 63-68-3 |
DL-Homocysteine |
Millipore Sigma |
Cat#: H4628, CAS: 454-29-5 |
Choline Chloride |
Millipore Sigma |
Cat#: C7017–5G, CAS:67-48-1 |
Levamisole hydrochloride |
Fisher Scientific |
Cat#: AC187870100 CAS: 16595-80-5 |
EDTA-free Protease Inhibitor cocktail |
Roche |
Cat#: 4693159001 |
NP-40 |
ThermoFisher Scientific |
Cat#: 85124, CAS: 9016-45-9 |
Sodium Dodecyl Sulfate |
Fisher Bioreagents |
Cat#: BP1311-200, CAS: 151-21-3,7732-18-5 |
Sodium Chloride (NaCl) |
Fisher Bioreagents |
Cat#: BP358-1 CAS:7647-14-5 |
1M Tris-HCl pH 8.3 |
Teknova |
Cat#: T5083 |
Tris Base |
Fisher Bioreagents |
Cat#: BP152-500, CAS: 77-86-1 |
TRIS-buffered saline (TBS, 10x) |
Alfa Aesar |
Cat#: J62938-K2, CAS: 77-86-1 |
Tween-20 |
Fisher Bioreagents |
Cat#: BP337-100 CAS: 9005-64-5 |
Non-Fat Powdered Milk |
Boston BioProducts |
Cat#: P-1400 |
β-meracaptoethanol |
Fisher Scientific |
Cat#: AC125470100 CAS: 60-24-2 |
Bromophenol Blue |
Fisher Scientific |
Cat#: AC403140050 CAS: 115-39-9 |
Urea |
ThermoFisher Scientific |
Cat#: 29700, CAS: 57-13-6 |
Glycerol |
Fisher Bioreagents |
Cat#: BP229-1, CAS: 56-81-5 |
10x Tris/Glycine/SDS Buffer |
BioRad |
Cat#: 1210732 |
Glycine |
Fisher Bioreagents |
Cat#: BP381-500 CAS:56-40-6 |
Methanol |
Fisher Chemical |
Cat#: A454-4, CAS: 67-56-1 |
16.5% Mini-Protean Tris-Tricine Gel |
BioRad |
Cat#: 4563063 |
10% Mini-Protean TGX Precast Protein Gels |
BioRad |
Cat#: 4561033 |
Trizol |
ThermoFisher Scientific |
Cat#: 15596018 |
Chloroform |
Crescent Chemical Co. |
Cat#: 39553.01, CAS: 67-66-3 |
RNase-Free DNase Set |
QIAGEN |
Cat#: 79254 |
PowerUp SYBR Green Master Mix |
Applied Biosystems |
Cat#: A25741 |
0.5M EDTA pH 8.0 |
Invitrogen |
Cat#: 15575-038 |
2′7’-Dichlorofluorescin diacetate |
Millipore Sigma |
Cat#: D6883-250, CAS: 4091-99-0 |
Nile Red |
ThermoFisher Scientific |
Cat#: N1142 |
Agarose |
Fisher Bioreagents |
Cat#:BP160-500, CAS: 9012-36-6 |
Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) |
Millipore Sigma |
Cat#: C2920-10, CAS: 370-86-5 |
DMSO (Dimethyl Sulfoxide) LC-MS Grade |
ThermoFisher Scientific |
Cat#: 85190, Cas:67-68-5 |
Sodium Azide |
Acros Organics |
Cat#: 19038-1000, CAS: 26629-22-8 |
Paraformaldehyde (16%) Solution EM Grade |
Electron Microscopy Sciences |
Cat#: 15710, CAS: 30525-89-4 |
Water, Molecular Grade, Sterile, DEPC Free |
Fisher Scientific |
Cat#: R91450001G, CAS: 7732-18-5 |
Critical commercial assays |
Pierce BCA Protein Assay Kit |
ThermoFisher Scientific |
Cat#: 23327 |
ATP Bioluminescence Assay Kit CLS II |
Roche Diagnostics |
Cat#: 11699695001 |
SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Scientific |
Cat#: 34580 |
iScript cDNA Synthesis Kit |
BioRad |
Cat#: 1708890 |
RNeasy Mini Kit |
QIAGEN |
Cat#: 74104 |
MitoSox Red Mitochondrial Superoxide Indicator |
ThermoFisher Scientific |
Cat#: M36008
|
Amplex red Hydrogen Peroxide/Peroxidase Assay Kit |
ThermoFisher Scientific |
Cat#: A22188 |
Homocysteine Fluorometric Kit |
Abcam |
Cat#: Ab228559
|
Seahorse XFe96 FluxPak |
Agilent |
Cat#: 102416-100 |
Seahorse XFe96 Cell Culture Microplate |
Agilent |
Cat#: 101085-004 |
Deposited data |
Mendeley Data |
This study |
https://doi.org/10.17632/djjv7tg4rv.1
|
Experimental models: Organisms/strains |
Caenorhabditis elegans (WT) |
CGC |
N2 |
dvIs100 [unc-54p::A-beta-1-42::unc-54 3′-UTR + mtl-2p::GFP] V |
CGC |
GMC101 |
smg-1(cc546) I; dvIs27 [myo-3p::A-Beta (1-42)::let-851 3′UTR) + rol-6(su1006)] X |
CGC |
CL4176 |
eat-2 (ad465) II; dvls100[unc-54p::A-beta-1-42 + mtl-2p::GFP] V |
This study |
UDE96 |
gcn-2(ok871) II; dvIs100 [unc-54p::A-beta-1-42:: unc-54 3′UTR + mtl-2p::GFP] V |
This study |
UDE90 |
dvIs100[unc-54p::A-beta-1-42::unc-54 3′UTR + mtl-2p::GFP] V; pek-1(ok275) X |
This study |
UDE21 |
foxSi16 [myo-3p::tomm-20:: mKate2::HA::tbb-2 3′ UTR] I |
CGC |
SJZ47 |
foxSi16 [myo-3p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I; dvls100 [unc-54p::A-beta-1-42 + mtl-2p::GFP] V |
This study |
UDE123 |
wwIs24 [acdh-1p::GFP + unc-119(+)]
|
CGC |
VL749 |
dvIs100[unc-54p::A-beta-1-42::unc-54 3′UTR + mtl-2p::GFP] V; pcca-1(ok2282) X |
This study |
UDE148 |
metr-1(ok521) II; dvIs100 [unc-54p::A-beta-1-42:: unc-54 3′UTR + mtl-2p::GFP] V |
This study |
UDE146 |
mmcm-1 (ok1637) III; dvls100[unc-54p:: A-beta-1-42 + mtl-2p::GFP] V |
This study |
UDE194 |
dvls100[unc-54p::A-beta-1-42 + mtl-2p:: GFP] V; sams-1 (ok2946) X |
This study |
UDE205 |
rmIs133[unc-54p::Q40::YFP] I |
CGC |
AM141 |
rmIs290 [unc-54p::Hsa-sod-1 (127X)::YFP] IV |
CGC |
AM725 |
Oligonucleotides |
eat-2(ad465) II |
eat-2 forward: GCTCATTCGCACTGTTGAA TAACACTTATAGCGGTTTCT |
Common Reverse: GTTACTTA AGGCGTACGAGCC |
Wild type at eat-2(ad465) II locus |
WT forward: GCTCATTCGCACTGTTGAATAACA CTTATAGC GGTTTCC |
Common Reverse: GTTACTTA AGGCGTACGAGCC |
gcn-2(ok871) II |
gcn-2 forward: GTGTTCACCGCTG AATCGG |
Common Reverse: GACCACA TCCATCGCAACAC |
Wild type at gcn-2(ok871) II locus |
WT forward: GCAGTGTTCTTCGCCTTCTC |
Common Reverse: GACCACA TCCATCGCAACAC |
pek-1(ok275) X |
pek-1 forward: CTCTTGACAGCGTACTCG |
Common Reverse: GCCAACA GTACATCGATG |
Wild type at pek-1(ok275) X locus |
WT forward: GACACCAATCTCAGCTGTAG |
Common Reverse: GCCAACA GTACATCGATG |
pcca-1(ok2282) X |
pcca-1 forward: CAGGTGAGGGTAATGGCATC |
Common Reverse: GCTGGA GCCAAGTTCATC |
Wild type at pcca-1(ok2282) X locus |
WT forward: CCTGAAGACGAGTGTTCATC |
Common Reverse: GCTGGA GCCAAGTTCATC |
metr-1(ok521) II |
metr-1 forward: GAGGAGTCGACGTG CTTCTC |
Common Reverse: CGTCCG ACAAAGGAAGGC |
Wild type at metr-1(ok521) II locus |
WT forward: GTCGTTGGAGAGCTGTTC |
COMMON REVERSE: CGTCCGA CAAAGGAAGGC |
mmcm-1(ok1637) III |
mmcm-1 forward: GATGGCATTCTTGG GACCAG |
Common Reverse: GAGAGACGC GGAGCAAATGC |
Wild type at mmcm-1(ok1637) III locus |
WT forward: GAGAGACGCGGAG CAAATGC |
Common Reverse: GAGAGAC GCGGAGCAAATGC |
sams-1(ok2946) X |
sams-1 forward: GGAGAAATTACCT CCAAGGC |
Common Reverse: GCGATAGC AGATGTGGCTGG |
Wild type at sams-1(ok2946) X locus |
WT forward: GGTCTCTACCCAACACTCTC |
Common Reverse: GCGATAGC AGATGTGGCTGG |
nuo-2 qRT-PCR primers |
GTGCTGACTGGTTTGAGC |
GACCTCGTTGTATCCCG |
mev-1 qRT-PCR primers |
GGACAGATCTACAAATCGGG |
CTTGTTGCTCTTGTTCTGGC |
cyc-1 qRT-PCR primers |
CGACATTGCTTCTGTACG |
CAGTCATGATTGTGTCAACG |
nduo-1 qRT-PCR primers |
GCCATCCGTGCTAGAAGAC |
CAAATGGCGCCCGGTTAAG |
act-2 qRT-PCR primers |
GCGCAAGTACTCCGTCTGGATCG |
GGGTGTGAAAATCCGTAAGGCAGA |
Software and algorithms |
ImageJ |
National Institutes of Health |
https://imagej.nih.gov/ij/
|
Graph Pad Prism 9 |
GraphPad Software Inc. |
https://www.graphpad.com/
|
Quantstudio6 Real-time PCR |
Thermofisher Scientific |
Cat: 4485694 |
ZEN 2.3 Digital Imaging System |
Zeiss |
https://www.zeiss.com/corporate/int/home.html?vaURL=www.zeiss.de/en
|