KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-β-Amyloid 6E10 | BioLegend | Cat#803001; RRID: AB_2564653 |
| anti-α-tubulin A11 | Millipore Sigma | Cat#T9026; RRID: AB_477593 |
| Anti-NDUFS3 | Abcam | Cat#ab14711; RRID: AB_301429 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP | ThermoFisher Scientific | Cat#31430; RRID: AB_228307 |
| Bacterial and virus strains | ||
| Escherichia coli OP50 | Caenorhabditis Genetics Center (CGC) | N/A |
| Escherichia coli HB101 | CGC | N/A |
| Escherichia coli HT115 | CGC | N/A |
| Escherichia coli DA837 | CGC | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| D(+)-glucose | Fisher Scientific | Cat#: AC410955000 CAS: 50-99-7 |
| Sodium Homogamma Linolenate | Nu-Chek Prep | Cat#: S-1143, CAS: 65881-87-0 |
| Oleic Acid | ThermoFisher Scientific | Cat#:31997, CAS: 112-80-1 |
| Methylcobalamin | Millipore Sigma | Cat#: M9756, CAS: 13422-55-4 |
| L-methionine | Fisher Scientific | Cat#: AC166160025 CAS: 63-68-3 |
| DL-Homocysteine | Millipore Sigma | Cat#: H4628, CAS: 454-29-5 |
| Choline Chloride | Millipore Sigma | Cat#: C7017–5G, CAS:67-48-1 |
| Levamisole hydrochloride | Fisher Scientific | Cat#: AC187870100 CAS: 16595-80-5 |
| EDTA-free Protease Inhibitor cocktail | Roche | Cat#: 4693159001 |
| NP-40 | ThermoFisher Scientific | Cat#: 85124, CAS: 9016-45-9 |
| Sodium Dodecyl Sulfate | Fisher Bioreagents | Cat#: BP1311-200, CAS: 151-21-3,7732-18-5 |
| Sodium Chloride (NaCl) | Fisher Bioreagents | Cat#: BP358-1 CAS:7647-14-5 |
| 1M Tris-HCl pH 8.3 | Teknova | Cat#: T5083 |
| Tris Base | Fisher Bioreagents | Cat#: BP152-500, CAS: 77-86-1 |
| TRIS-buffered saline (TBS, 10x) | Alfa Aesar | Cat#: J62938-K2, CAS: 77-86-1 |
| Tween-20 | Fisher Bioreagents | Cat#: BP337-100 CAS: 9005-64-5 |
| Non-Fat Powdered Milk | Boston BioProducts | Cat#: P-1400 |
| β-meracaptoethanol | Fisher Scientific | Cat#: AC125470100 CAS: 60-24-2 |
| Bromophenol Blue | Fisher Scientific | Cat#: AC403140050 CAS: 115-39-9 |
| Urea | ThermoFisher Scientific | Cat#: 29700, CAS: 57-13-6 |
| Glycerol | Fisher Bioreagents | Cat#: BP229-1, CAS: 56-81-5 |
| 10x Tris/Glycine/SDS Buffer | BioRad | Cat#: 1210732 |
| Glycine | Fisher Bioreagents | Cat#: BP381-500 CAS:56-40-6 |
| Methanol | Fisher Chemical | Cat#: A454-4, CAS: 67-56-1 |
| 16.5% Mini-Protean Tris-Tricine Gel | BioRad | Cat#: 4563063 |
| 10% Mini-Protean TGX Precast Protein Gels | BioRad | Cat#: 4561033 |
| Trizol | ThermoFisher Scientific | Cat#: 15596018 |
| Chloroform | Crescent Chemical Co. | Cat#: 39553.01, CAS: 67-66-3 |
| RNase-Free DNase Set | QIAGEN | Cat#: 79254 |
| PowerUp SYBR Green Master Mix | Applied Biosystems | Cat#: A25741 |
| 0.5M EDTA pH 8.0 | Invitrogen | Cat#: 15575-038 |
| 2′7’-Dichlorofluorescin diacetate | Millipore Sigma | Cat#: D6883-250, CAS: 4091-99-0 |
| Nile Red | ThermoFisher Scientific | Cat#: N1142 |
| Agarose | Fisher Bioreagents | Cat#:BP160-500, CAS: 9012-36-6 |
| Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) | Millipore Sigma | Cat#: C2920-10, CAS: 370-86-5 |
| DMSO (Dimethyl Sulfoxide) LC-MS Grade | ThermoFisher Scientific | Cat#: 85190, Cas:67-68-5 |
| Sodium Azide | Acros Organics | Cat#: 19038-1000, CAS: 26629-22-8 |
| Paraformaldehyde (16%) Solution EM Grade | Electron Microscopy Sciences | Cat#: 15710, CAS: 30525-89-4 |
| Water, Molecular Grade, Sterile, DEPC Free | Fisher Scientific | Cat#: R91450001G, CAS: 7732-18-5 |
| Critical commercial assays | ||
| Pierce BCA Protein Assay Kit | ThermoFisher Scientific | Cat#: 23327 |
| ATP Bioluminescence Assay Kit CLS II | Roche Diagnostics | Cat#: 11699695001 |
| SuperSignal West Pico PLUS Chemiluminescent Substrate | Thermo Scientific | Cat#: 34580 |
| iScript cDNA Synthesis Kit | BioRad | Cat#: 1708890 |
| RNeasy Mini Kit | QIAGEN | Cat#: 74104 |
| MitoSox Red Mitochondrial Superoxide Indicator | ThermoFisher Scientific | Cat#: M36008 |
| Amplex red Hydrogen Peroxide/Peroxidase Assay Kit | ThermoFisher Scientific | Cat#: A22188 |
| Homocysteine Fluorometric Kit | Abcam | Cat#: Ab228559 |
| Seahorse XFe96 FluxPak | Agilent | Cat#: 102416-100 |
| Seahorse XFe96 Cell Culture Microplate | Agilent | Cat#: 101085-004 |
| Deposited data | ||
| Mendeley Data | This study | https://doi.org/10.17632/djjv7tg4rv.1 |
| Experimental models: Organisms/strains | ||
| Caenorhabditis elegans (WT) | CGC | N2 |
| dvIs100 [unc-54p::A-beta-1-42::unc-54 3′-UTR + mtl-2p::GFP] V | CGC | GMC101 |
| smg-1(cc546) I; dvIs27 [myo-3p::A-Beta (1-42)::let-851 3′UTR) + rol-6(su1006)] X | CGC | CL4176 |
| eat-2 (ad465) II; dvls100[unc-54p::A-beta-1-42 + mtl-2p::GFP] V | This study | UDE96 |
| gcn-2(ok871) II; dvIs100 [unc-54p::A-beta-1-42:: unc-54 3′UTR + mtl-2p::GFP] V | This study | UDE90 |
| dvIs100[unc-54p::A-beta-1-42::unc-54 3′UTR + mtl-2p::GFP] V; pek-1(ok275) X | This study | UDE21 |
| foxSi16 [myo-3p::tomm-20:: mKate2::HA::tbb-2 3′ UTR] I | CGC | SJZ47 |
| foxSi16 [myo-3p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I; dvls100 [unc-54p::A-beta-1-42 + mtl-2p::GFP] V | This study | UDE123 |
| wwIs24 [acdh-1p::GFP + unc-119(+)] | CGC | VL749 |
| dvIs100[unc-54p::A-beta-1-42::unc-54 3′UTR + mtl-2p::GFP] V; pcca-1(ok2282) X | This study | UDE148 |
| metr-1(ok521) II; dvIs100 [unc-54p::A-beta-1-42:: unc-54 3′UTR + mtl-2p::GFP] V | This study | UDE146 |
| mmcm-1 (ok1637) III; dvls100[unc-54p:: A-beta-1-42 + mtl-2p::GFP] V | This study | UDE194 |
| dvls100[unc-54p::A-beta-1-42 + mtl-2p:: GFP] V; sams-1 (ok2946) X | This study | UDE205 |
| rmIs133[unc-54p::Q40::YFP] I | CGC | AM141 |
| rmIs290 [unc-54p::Hsa-sod-1 (127X)::YFP] IV | CGC | AM725 |
| Oligonucleotides | ||
| eat-2(ad465) II | eat-2 forward: GCTCATTCGCACTGTTGAA TAACACTTATAGCGGTTTCT | Common Reverse: GTTACTTA AGGCGTACGAGCC |
| Wild type at eat-2(ad465) II locus | WT forward: GCTCATTCGCACTGTTGAATAACA CTTATAGC GGTTTCC | Common Reverse: GTTACTTA AGGCGTACGAGCC |
| gcn-2(ok871) II | gcn-2 forward: GTGTTCACCGCTG AATCGG | Common Reverse: GACCACA TCCATCGCAACAC |
| Wild type at gcn-2(ok871) II locus | WT forward: GCAGTGTTCTTCGCCTTCTC | Common Reverse: GACCACA TCCATCGCAACAC |
| pek-1(ok275) X | pek-1 forward: CTCTTGACAGCGTACTCG | Common Reverse: GCCAACA GTACATCGATG |
| Wild type at pek-1(ok275) X locus | WT forward: GACACCAATCTCAGCTGTAG | Common Reverse: GCCAACA GTACATCGATG |
| pcca-1(ok2282) X | pcca-1 forward: CAGGTGAGGGTAATGGCATC | Common Reverse: GCTGGA GCCAAGTTCATC |
| Wild type at pcca-1(ok2282) X locus | WT forward: CCTGAAGACGAGTGTTCATC | Common Reverse: GCTGGA GCCAAGTTCATC |
| metr-1(ok521) II | metr-1 forward: GAGGAGTCGACGTG CTTCTC | Common Reverse: CGTCCG ACAAAGGAAGGC |
| Wild type at metr-1(ok521) II locus | WT forward: GTCGTTGGAGAGCTGTTC | COMMON REVERSE: CGTCCGA CAAAGGAAGGC |
| mmcm-1(ok1637) III | mmcm-1 forward: GATGGCATTCTTGG GACCAG | Common Reverse: GAGAGACGC GGAGCAAATGC |
| Wild type at mmcm-1(ok1637) III locus | WT forward: GAGAGACGCGGAG CAAATGC | Common Reverse: GAGAGAC GCGGAGCAAATGC |
| sams-1(ok2946) X | sams-1 forward: GGAGAAATTACCT CCAAGGC | Common Reverse: GCGATAGC AGATGTGGCTGG |
| Wild type at sams-1(ok2946) X locus | WT forward: GGTCTCTACCCAACACTCTC | Common Reverse: GCGATAGC AGATGTGGCTGG |
| nuo-2 qRT-PCR primers | GTGCTGACTGGTTTGAGC | GACCTCGTTGTATCCCG |
| mev-1 qRT-PCR primers | GGACAGATCTACAAATCGGG | CTTGTTGCTCTTGTTCTGGC |
| cyc-1 qRT-PCR primers | CGACATTGCTTCTGTACG | CAGTCATGATTGTGTCAACG |
| nduo-1 qRT-PCR primers | GCCATCCGTGCTAGAAGAC | CAAATGGCGCCCGGTTAAG |
| act-2 qRT-PCR primers | GCGCAAGTACTCCGTCTGGATCG | GGGTGTGAAAATCCGTAAGGCAGA |
| Software and algorithms | ||
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| Graph Pad Prism 9 | GraphPad Software Inc. | https://www.graphpad.com/ |
| Quantstudio6 Real-time PCR | Thermofisher Scientific | Cat: 4485694 |
| ZEN 2.3 Digital Imaging System | Zeiss | https://www.zeiss.com/corporate/int/home.html?vaURL=www.zeiss.de/en |