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FAP activity but not DPP IV activity is absent in Fap−/− embryos. (A) FAP-specific DPP activity assay. (B) DPP IV-specific DPP activity assay. Detergent-soluble membrane extracts obtained from two Fap+/+ and Fap−/− embryos (E17.5) were immunoprecipitated with MAb 3D11 (A) and with anti-CD26 MAb H194-112 (B), and different dilutions (indicated) of the immunoprecipitates were used in the DPP assay.
