TABLE 2.
LC-MS/MS proteomic comparisons of porin proteins, OmpA, and DegP abundance in E. coli isolates versus cefalexin-resistant mutant derivativesa
| Isolate or mutant | OmpF |
OmpC |
OmpA |
DegP |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SEM | P (WT/M) | Mean | SEM | P (WT/M) | Mean | SEM | P (WT/M) | Mean | SEM | P (WT/M) | |
| PSA | 0.23 | 0.05 | 5.93 | 1.00 | 3.24 | 0.32 | 0.06 | 0.01 | ||||
| PSA (M) | 0.00 | 0.00 | 0.005 | 0.17 | 0.04 | 0.002 | 3.69 | 0.74 | >0.25 | 0.03 | 0.01 | 0.03 |
| ATCC 25922 | 1.69 | 0.10 | 1.66 | 0.26 | 5.55 | 0.41 | 0.08 | 0.01 | ||||
| ATCC 25922 (M) | 0.29 | 0.14 | 0.0006 | 0.24 | 0.05 | 0.003 | 1.00 | 0.17 | 0.0003 | 0.54 | 0.10 | 0.005 |
| EC17 | 1.15 | 0.15 | 2.49 | 0.50 | 3.79 | 0.29 | 0.17 | 0.03 | ||||
| EC17 (M) | 0.39 | 0.16 | 0.01 | 2.25 | 0.66 | >0.25 | 4.47 | 0.78 | >0.25 | 0.74 | 0.10 | 0.003 |
a
Abundance relative to total ribosomal proteins is presented for each protein; n = 3 biological replicates for each wild-type/mutant pair (WT/M). Raw data are presented in Tables S1 to S3 in the supplemental material.